Skunk Pharm Research LLC Research and Development one safety meeting at a time

Sorry to take so long to get back to ya’ll asking to purchase a Terpenator, but we had to take the extra step of discussing it with our business attorney.

Yes, we will take care of all of ya’ll.  For the mechanically handy, I will publish the complete details of the current Mk II as I put it together, and am available to hold your hand and field any questions you might have as you build and learn to use your own DIY unit.

For those who want to purchase a unit or critical component kits for a unit, Skunk PharmResearch, LLC’s bylaws proscribe doing retail sales, or business outside the state of Oregon.

However, due to the number of inquiries, after discussing it with our attorney and a couple of the businesses that we lease equipment to, we are going to license our technology to a company who does do retail sales nationally and internationally.

More on that, after they have had an opportunity to get set up.  In the interim, please enjoy this resource site, which will remain free to all and uncluttered with advertisements, because retail sales will remain outside our core purpose.

Until 2007 there were no published reports of highly successful methods for culturing Cannabis sativa. Hemp was successfully cultured in China but at a success rate of a mere 85%. Then Ole Miss published “Thidiazuron-induced high-frequency direct shoot organogenesis of Cannabis sativa L.” in The Society for In Vitro Biology which detailed how they achieved a 95% success rate.

We found this paper and started making the medium. We were able to get great results with the vegetative propagation medium which utilizes hormones TDZ and gibbrellic acid. We used this medium to multiply and expand our library. The rooting medium calls for half strength MS salts and the exchange of hormones to only IBA and with the addition of activated charcoal. We had problems initially with the medium being watery after sterilization but we have since linked the issue to our gelling agent and have moved forward.

Synthetic seeds

During our research we also came across another paper published by Ole Miss on synthetic seeds. These same scientists were able to develop a formulation that consistently would regenerate 100% of these synthetic seeds under greenhouse conditions. Impressive statistics, but not an original or groundbreaking discovery; synthetic seeds have been around a while and are underutilized technology.

We have also been tooling around in our spare time with synthetic seeds and have some preliminary observations on their implementation.

Our first batch of synthetic seeds.

For the rest of this article see:

http://skunkpharmresearch.com/tissue-culture/

After pricing having our samples run at an outside lab, we decided to cut to the chase and managed to pick up a used Perkins Elmer 8500 Gas Chromatograph from a Christian university back east, replete with software, logs, manuals, and both FID and hot wire detectors.

A gorgeous thang, in pristine condition and according to the used equipment dealer, had only been used to test Holy Water, and then only on Sundays.  We immediately admired that about it, and plan to use it to test our Holy Shit sublingual potion as well, plus some other thangs.

Of course after buying it, we immediately had to kick out a wart on our newly framed skunk shack lab to have a purdy place to set it, and I’ve been taking bids on the required Hydrogen carrier gas.

It is proving to be an interesting project, as our new GC was actually built in 1987, so we are out digging for a large floppy drive, a 386 serial port computer, and a dot matrix serial port printer.

As fate would have it, I have my original computer in the attic, which just happens to be a 386 and was working fine when I replaced it, and an old Panasonic dot matrix printer.  Hee, hee, hee………………..

It is amazing how much computer technology has changed since 1987, so we are also investigating ways to interface with a modern computer.

Mostly we are looking forward to finally attaching empirical numbers to the anecdotal evidence from volunteer patient test panels.

Ahwoooooooooooooooooooooooooooooooooooooooooooo!!!!!!!!!!!!!!!!!!!1

Our New Skunk Shack and Lab! 

Hi ya’ll!  Please excuse our sites construction mess!  We’uns are just learning to drive this puppy!

Our site isn’t the only thing that is a mess either.  As our business plan doesn’t involve high cash flow, we elected to not pay to have an unneeded store front.  We did however decide to move our operation out of my garage and the pop up canopy in my courtyard.

What we are doing, is building a 12′ X 12′ skunk shack and lab at another location, isolated by itself on a slab.  We will have a controlled drying chamber, a closed and ventilated extraction cabinet for running flammable extractions, certified flammable liquid storage,  a lab bench, a gas chromatograph.  It will also be air-conditioned, and a has a kitchenette with stove top, sink, and fridge.

We currently have it poured, framed, skinned, and roofed.  We still need to wire it, insulate and drywall it, and side the outside over the 3/4″ plywood exterior sheeting.

Construction is 2X4 except around the door area, which is 2X6, with a reinforced door, and multi loop alarm system.

We are through framing except for installing the last pre-hung interior door, and wiring is our next step.  Since we didn’t cast our conduit in the slab pour, we will rent a concrete core drill to punch through the slab, and lay the electrical service run in a trench, along with the water and waste return lines.

The waste return line will be pumped, as no sewer lines are available  that far from the main building.  We won’t put it into the sewer under pressure, but will instead pump in into a catch tank, with an open drain to the sewer.

Besides a small lab, we will have a kitchen unit, flammable liquid storage cabinet, a plant drying chamber, a ventilated extraction cabinet, and space for our new used gas chromatograph.  That is a bunch in 144 square feet.

Watch this space for updates and comments!

Lola guarding new skunk shack until door is installed!

Donated cabinets, with new top and shelves!

Space for Gas Chromatograph and kitchenette.

Drying closet on left and extraction cabinet on right.

7-27-12

Hee, hee, hee, we have security!  Here is our steel clad front door, built from scratch, including the steel jamb.  Awhoooooooooooooooooooo!!!!!!1

Lights are on 8-2-12

GW